1. Take the vial out of the fridge and let it come to room temperature. Cold glass and cold water make the powder harder to dissolve.
2. Flick the vial gently so any powder clinging to the walls settles at the bottom.
3. Swab the rubber stopper on both the peptide vial and the bacteriostatic water, and let the alcohol dry rather than wiping it.
4. Draw your bacteriostatic water, then put the needle in at an angle so the water runs down the inside wall of the glass. Squirting it straight onto the powder damages it.
5. Swirl or roll it. Never shake it. Shaking shears the peptide. Give it a few minutes if it doesn't go clear straight away.
6. It should finish completely clear with nothing floating in it.

How much water you add is your choice — it changes nothing about how much peptide is in the vial, only how many units on the syringe a given amount works out to. The Mix tab does that arithmetic.

Before mixing — the freeze-dried powder is the stable form. Keep it cold and out of the light. It tolerates room temperature in transit, which is why it can be posted.

After mixing — back in the fridge, 2–8 °C, upright, away from the door where the temperature swings. Don't freeze a reconstituted vial.

Light and heat are what degrade it. The fridge door and a sunny windowsill are the two places it shouldn't be.

You'll see 28 days quoted everywhere for a reconstituted vial. It's worth knowing what that number is, because it is repeated as though it were data and it isn't.

28 days is a handling convention for preserved multi-dose vials. It rests on the benzyl alcohol in bacteriostatic water and on how many times the stopper has been punctured. It's about keeping the vial sterile.

It is not a measurement of the peptide breaking down. That's a separate question, answered differently for every compound, and often a good deal longer when refrigerated.

The industry answers both questions with one number, and that's the flaw. We recommend 28 days — it's what the tracker counts to. Sterility and stability are two different clocks, and we're not going to tell you a longer figure is fine, because nobody's anecdote is evidence.

Your syringes are U-100. That means 100 units is one full millilitre, so one unit is 0.01 mL. Units are just a volume marking — they say nothing on their own about how much peptide you've drawn. That depends entirely on how much water you mixed with.

• Draw slowly. Pulling hard makes bubbles.
• Tap the barrel to float bubbles to the needle end and push them back out before you read the mark.
• Read at the flat front edge of the plunger seal, at eye level.
• Very small draws are the hard ones. Below about two units the marks are too coarse to read honestly — mixing with more water spreads the same amount over more units and makes it readable. The Mix tab warns you when you're in that territory.

• Swab the stopper every single time, not just the first time.
• A fresh syringe for every draw. Reusing one blunts the needle and is the fastest way to contaminate a vial.
• Never touch the needle to anything before it goes through the stopper.
• Sharps go in a proper sharps container. Most Alberta pharmacies will take a full one back.

Using the same spot repeatedly is what causes trouble — the tissue thickens and hardens, it gets sore, and once that happens absorption from that patch stops being predictable. The fix is simply not to go back to the same place twice in a row.

The app keeps track for you. When you log a dose it shows a body map with anywhere you've used in the last week shaded, and picks out whichever spot has been left alone longest. Tap a different one if you'd rather — it records what you choose, it doesn't insist.

Give a spot at least a week before returning to it, and move a couple of centimetres even within the same area. If somewhere is sore, lumpy, bruised or hard, leave it alone until it's normal again.

A reconstituted vial should be clear. Stop and get in touch if you see cloudiness, floating particles, a colour change, or a stopper that's come loose or looks damaged.

Don't try to rescue it by filtering it or mixing more water in. Photograph it and email info@sentientbiogenix.com.

Water, syringes and swabs, straight from us. Worth keeping a spare of each — running out of bacteriostatic water mid-protocol is the usual reason people get stuck.

Everything Sentient Biogenix sells is supplied for laboratory research. That isn't a formality we tack on the bottom of an invoice — it's what the products are, and it sets what we're able to help with.

We can help with: handling, mixing, storage, syringes and technique, what's in a vial, purity and certificates, and anything to do with your order.

We can't help with: what to take, how much, how often, how long for, or how to combine things. Not because we're being cagey — we aren't qualified to, and anyone in our position telling you otherwise should worry you.

If you want that kind of guidance, it needs to come from a healthcare professional who knows your situation. This app is a notebook that does arithmetic. It records what you tell it and nothing more.